Cell sorting by Tsl1-GFP content alters growth rate distributions and heat shock survival.

<p>(A) The distribution of Tsl1 abundance does not appear bimodal. Histogram of single-cell Tsl1-GFP intensity as measured by fluorescence-activated cell sorting. Inset: the right-hand tail of the main figure. Cells with the top 0.1% (dark green) and next 0.1 to 1% (light green) Tsl1-GFP fluorescence were sorted for downstream analysis. (B) Survival of sorted populations following heat shock of a liquid suspension as measured by plating on agar plates. <i>p</i>-Values are a comparison to the unsorted population (purple), Student's <i>t</i> test of arcsin transformed data: *, <i>p</i><0.01; **, <i>p</i><1×10⁻⁵. Error bars indicate SEM. (C) Sorting for Tsl1-GFP abundance transiently alters growth rate distributions. Left: cumulative growth rate distributions of sorted cells (dark and light green), cells passed through the cell sorter but unsorted (purple), and cells not passed through the cell sorter (orange). Right: Samples of the same sorted or unsorted cell populations after ∼42 generations of growth.