Expression of a dominant-negative form of Hsp70 influences the persistence of Cyc8^4KtoR inclusions.

<p>(<b>A</b>) Cells expressing Cyc8^4KtoR-GFP from the endogenous <i>CYC8</i> locus and carrying either an empty 2 micron vector or a 2 micron vector with an dominant-negative form of Hsp70 (<i>SSA1</i>) were exposed to a time course of hyperosmotic stress (1.2M sorbitol), fixed at the times indicated, and imaged by fluorescence microscopy. (<b>B</b>) Analysis of data from (A). Six fields of cells for each condition, with at least 40 cells/field, were counted for the presence of cytoplasmic inclusions. Data represent the average percentage of cells that contained cytoplasmic inclusions within the six fields. Error bars are the standard deviation. A two-tailed, heteroscedastic student’s t-test was used to determine significance.