Aberrant Hsp70 localization upon heat shock in cells expressing polyQ-expanded Htt.

<p><b>A</b>) Immunofluorescence microscopy of fixed cells that were grown at 33°C and heat shocked cells (HS, 42°C) for either one, three, or six hours probed with anti-Hsp70 antibodies. The scale bars represent 75 µm. The 33°C samples were exposed longer to visualize Hsp70 localization. <b>B</b>) Immunofluorescence microscopy of fixed cells as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037929#pone-0037929-g004" target="_blank">Figure 4 A</a>. Pictures were taken at a higher magnification (scale bars represent 15 µm) to demonstrate the difference between diffuse and granular Hsp70 staining in the nucleus.