Rostral migratory stream in the dog brain.

<p>(A) Schematic of a sagittal view of a canine brain. The red line indicates the location of the RMS in relationship to the anterior horm of the lateral ventricle (LV), caudate nucleus (CN), olfactory bulb (OB), cortex (Ctx) and cerebellum (Cb). (B) Nissl staining showing the orientation and nomenclature of the canine RMS. (C) anti-BrdU immunostaining in brown with hematoxylin counterstain in purple. C1 shows BrdU staining at the boundary of the white matter and caudate nucleus in the descending limb; C2 shows BrdU staining in the olfactory peduncle/rostral limb. (D) anti-Dcx immunostaining. D1 shows morphology of cells in the funnel; D2 shows leading and trailing processes of migrating cells in the descending limb. (E) Immunoreactivity for BrdU (green, in the RMS) and NeuN (red, in the CN) does not overlap in the descending limb. Section is counterstained with DAPI (blue). (F) BrdU immunostaining (brown) in the olfactory peduncle in tissue from dog 5, analyzed at 6 hr after a single 75 mg/kg i.v., indicating that BrdU is taken up by dividing cells all along the RMS. Scale bar in B, C, D: 1 mm. Scale bars in C1, C2, E: 100 microns. Scale bars in D1, D2, F: 50 microns. Please view the figures on a computer monitor for accurate RGB color representation.