Effect of GATC mutations on <i>STM2209-STM2208</i> expression.

<p><b>A.</b> Diagram of the promoter region of <i>STM2209-STM2208</i>, showing GATC sites (red squares), putative OxyR-binding-sites (orange bars), putative −35 and −10 modules (green boxes) and the transcription initation site (black arrow). <b>B.</b> Effect of eliminating the 4 GATC sites upstream the <i>STM2209-STM2208</i> promoter on <i>STM2209-STM2208</i> expression, monitored by comparing the ß-galactosidase activity of a translational <i>STM2208::lac</i> fusion in Dam⁺ and Dam⁻ backgrounds (white and black histograms, respectively). Values are averages and standard deviations from 6 independent experiments. <b>C.</b> Effect of eliminating the 4 GATC sites upstream the <i>STM2209-STM2208</i> promoter on <i>STM2209-STM2208</i> expression, monitored by Western blot analysis of STM2208-3xFLAG levels in different backgrounds.