<i>mbo</i> operon transcriptional characterisation.

<p>(<b>A</b>) Schematic representation of the location and size of the amplified region obtained during the RT-PCR experiments. (<b>B</b>) PCR products from RT-PCR experiments using genomic DNA and mRNA as templates obtained from wild-type UMAF0158 and processed after 48 h of incubation at 22°C on liquid minimal medium PMS. The primer pairs used is detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036709#pone.0036709.s006" target="_blank">Table S2</a>. (<b>C</b>) Northern blot analysis of total RNA obtained from wild-type UMAF0158 and mutants in <i>gacA</i> and <i>mgoA</i> genes, using a DNA fragment between <i>mboC</i> and <i>mboD</i> genes as probes (shown in red in panel A). Lane M, ssRNA ladder; lane 1, UMAF0158; lane 2, <i>gacA</i><b><i>⁻</i></b> (UMAF0158-2βB7) and lane 3, <i>mgoA</i><b><i>⁻</i></b>.