Transcriptional analysis of putative <i>sca</i> operons by RT-PCR.

<p>RNA was extracted from 72 h-infected L929 fibroblasts and RT-PCR with primers designed to amplify the regions indicated in (A). A) Schematic of putative <i>sca</i> operons. Blue bars indicate regions successfully amplified, red bars indicate regions that could not be amplified. B) RT-PCR amplification of regions between ORFs in putative <i>sca</i> operons. Left panel – lanes 2 thru 4 – RT-PCR amplification of the 400 bp region within the encoded passenger domain of <i>sca3</i> used in time course analyses ; lanes 5 thru 7 – amplification of <i>Lon-sca3</i> co-transcript. Right panel: lanes 2 thru 4 – amplification of <i>sca4</i> region used in time course analyses; lanes 5 thru 7 – amplification of <i>tlcD-sca4</i> transcript. C) Analysis of <i>sca5</i> operon; labels indicate genes spanned by primers. RT – reverse transcriptase reaction, NRT – no reverse transcriptase, D – DNA control.