Spectral shift and apoShp titration in heme transfer from holoShr to apoShp.

<p>(<b>A</b>) Comparison of the absorption spectrum of 3.2 µM holoShr alone with the spectrum of a mixture of 3.2 µM holoShr and 23 µM apoShp. The spectrum of the mixture was taken immediately after mixing, and the spectrum of 3.2 µM holoShp was included for comparison. (<b>B</b>) ApoShp titration in heme transfer from holoShr to apoShp. Shown are the A₂₈₀/A₄₀₈ ratio of the recovered Shr and the amount of transferred heme.