Cells encoding <i>IME4</i> mutant alleles show developmental defects.

<p>A) FACS analysis of DNA synthesis in wild-type (SAy821), <i>ime4-cat/cat</i> (SAy1086) and <i>ime4</i>Δ/Δ (SAy771) strains throughout a meiotic time course (n = 3×10⁴ cells/strain/time point). DNA content of diploid cells before DNA replication (2C) and after DNA replication (4C) is indicated. B) <i>NDT80</i> transcript levels in the strains from (A) during a meiotic time course. Transcript levels were determined by RT-PCR and normalized to <i>ACT1</i> transcript levels. C) Kinetics for meiotic nuclear divisions as assayed by DAPI DNA staining in the strains from (A) (n = 200 cells/strain). D) Number of asci with one, two, three, four, or no spores in the strains from (A) after 24 hours in SPO medium (n = 200 cell/strain). E) Spore viability in the strains from (A); legend indicates number of surviving spores upon dissection (n = 187 tetrads/strain). F) Representative images of cells from strains in (A) after RTG₃ (top panels—arrows indicate primary buds) and colonies grown on SLAD for 6 days (bottom panels). G) Quantification of axial ratios of RTG₃ cells shown in (F), (n = 200 cells/strain). H) Representative images of cells after RTG₃ (top panels—arrows indicate primary buds) and colonies grown on SLAD for 6 days (bottom panels) of wild-type (SAy821), <i>ime2</i>Δ/Δ (SAy859), <i>ime4</i>Δ/Δ (SAy771) or <i>ime2</i>Δ/Δ <i>ime4</i>Δ/Δ (SAy1123) strains. Arrows indicate primary daughter cells. I) Quantification of axial ratios of RTG₃ cells shown in (H), (n = 200 cells/strain).