<p>Isogenic diploids carrying different copy numbers of <i>IME1</i> were shifted to meiotic inducing conditions. <b>A.</b> The correlation between <i>IME1</i> copy number and its maximal level of expression. The maximal level of <i>IME1</i> RNA (from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0011005#pone-0011005-g003" target="_blank">Fig. 3A</a>) in comparison to the copy number of the <i>IME1</i> gene was drawn. The fitted trend line (dotted) and its formula are given. <b>B.</b> Efficiency of meiosis. The percentage of asci (line) and the number of cells that can form colonies (viability, column) at 48 hours in SPM. The results are the averages of three independent transformants and a count of ≥200 cells. Error ...
Meiosis reduces the ploidy of the genome to generate haploid gametes for sexual reproduction. As gam...
<p>Note: The progression of meiosis was evaluated at the end of 18-hour culture. The number of oocyt...
<p>A) m<sup>6</sup>A accumulation on mRNA was quantified in rich conditions in wild-type (SAy821), <...
<p>Isogenic diploids carrying 1 (Y1639, diamonds), 2 (Y1631, squares), 3 (Y1663, triangles) and 5 (Y...
<p>Isogenic diploids carrying different copy numbers of <i>IME1</i> were shifted to meiotic conditio...
<p>Wild type diploid cells (Y1631) were shifted to meiotic conditions (sporulation medium, SPM), and...
<p>A) Western analysis for co-immunoprecipitation of Mum2 (left panels) and Slz1 (right panels) with...
<p>A) Western analysis for 3x-myc-tagged Ime4 protein (SAy914) throughout meiosis; Pgk1 protein serv...
<p>A) FACS analysis of DNA synthesis in wild-type (SAy821), <i>ime4-cat/cat</i> (SAy1086) and <i>ime...
<p>The isogenic diploid strains <i>ime1</i>Δ<i>/IME1</i> (Y1639, filled squares) and <i>ime1</i>Δ<i>...
<p>(A–F) Percentage of cells that have completed at least the first meiotic division (post-MI) in in...
<p>A: FACS analysis of DNA content in wild-type and <i>trf4</i>Δ mutants taken at 2 hour time-points...
<p>(A) Western blot of depletion of 3HA-Smc5 (Y941) and 3HA-Nse4 (Y942) protein levels under the <i>...
(A) and (B) Crosses of h- ade6-M26 x h+ ade6-3049 were performed in MEA and plated for recombinant f...
<p>(<b>A</b>) Set-up of the soft-shift method. Cells were shifted to non-permissive temperature (33°...
Meiosis reduces the ploidy of the genome to generate haploid gametes for sexual reproduction. As gam...
<p>Note: The progression of meiosis was evaluated at the end of 18-hour culture. The number of oocyt...
<p>A) m<sup>6</sup>A accumulation on mRNA was quantified in rich conditions in wild-type (SAy821), <...
<p>Isogenic diploids carrying 1 (Y1639, diamonds), 2 (Y1631, squares), 3 (Y1663, triangles) and 5 (Y...
<p>Isogenic diploids carrying different copy numbers of <i>IME1</i> were shifted to meiotic conditio...
<p>Wild type diploid cells (Y1631) were shifted to meiotic conditions (sporulation medium, SPM), and...
<p>A) Western analysis for co-immunoprecipitation of Mum2 (left panels) and Slz1 (right panels) with...
<p>A) Western analysis for 3x-myc-tagged Ime4 protein (SAy914) throughout meiosis; Pgk1 protein serv...
<p>A) FACS analysis of DNA synthesis in wild-type (SAy821), <i>ime4-cat/cat</i> (SAy1086) and <i>ime...
<p>The isogenic diploid strains <i>ime1</i>Δ<i>/IME1</i> (Y1639, filled squares) and <i>ime1</i>Δ<i>...
<p>(A–F) Percentage of cells that have completed at least the first meiotic division (post-MI) in in...
<p>A: FACS analysis of DNA content in wild-type and <i>trf4</i>Δ mutants taken at 2 hour time-points...
<p>(A) Western blot of depletion of 3HA-Smc5 (Y941) and 3HA-Nse4 (Y942) protein levels under the <i>...
(A) and (B) Crosses of h- ade6-M26 x h+ ade6-3049 were performed in MEA and plated for recombinant f...
<p>(<b>A</b>) Set-up of the soft-shift method. Cells were shifted to non-permissive temperature (33°...
Meiosis reduces the ploidy of the genome to generate haploid gametes for sexual reproduction. As gam...
<p>Note: The progression of meiosis was evaluated at the end of 18-hour culture. The number of oocyt...
<p>A) m<sup>6</sup>A accumulation on mRNA was quantified in rich conditions in wild-type (SAy821), <...