Results from peptides spanning the cleavage sites 1711, 1972, 2172, and 2420.

<p>The plots show data from sets of overlapping 10-mer peptides representing portions of the HCV polyprotein where protease activity was detected at all three time points in our assay (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037441#pone-0037441-g003" target="_blank">Fig. 3</a>). Red dotted lines mark cutoffs computed from the maximum transformed Z-score in the negative direction. The Y–axis represents log-transformed p-values with the sign showing directionality. Z-scores were transformed using -sign(z)x10xLog10(Pz) where Pz is the p-value derived from standard normal distribution. Because the peptide sequences are shifted in increments of 1 amino acid, several adjacent peptides contain sufficient recognition sequences to be cleaved. Peptide sequences are written vertically and the HCV NS3/4A protease recognition sequences corresponding to identified P4-P4’ positions are shown in red.