Comparison amplification efficiency between nested PCR and standard PCR for three long NPCL markers.

<p>Each long NPCL marker was amplified in two contiguous and overlapping fragments (F1 and F2). Three different color cells are used to represent the agarose gel electrophoretic images of PCR products. For complete species names, please refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039256#pone.0039256.s006" target="_blank">Table S1</a>.