Generation and characterization of a new SMA-iPSC line.

<p>(<b>A</b>) Bright field images of the 77iSMA-e.12 SMA line showing typical pluripotent stem cell colony morphology, made by a combination of episomal vectors. Immunostaining shows expression of embryonic stem cell surface antigen SSEA4, Tra-1-60, Tra-1-81, and nuclear Oct4. (<b>B</b>) Quantitative RT–PCR analyses of <i>OCT4, SOX2, NANOG, cMYC, KLF4, LIN28</i> expression in seven clones of 77iSMA iPSCs relative to H1 hESCs. “Endogenous” indicates that primers were included in the 3′ untranslated region (UTR) measure expression of the endogenous gene only, whereas “total” indicates that primers in coding regions measure expression of both the endogenous gene and the transgene if present<b>.</b> Gene expression differences were not significant (ns) by one-way ANOVA and data are represented as mean ± SD. (<b>C</b>) 77iSMA and 13iSMA iPSCs show the expected lack of <i>SMN1</i> expression and maintenance of <i>SMN2</i> expression. (<b>D</b>) Hematoxylin and eosin (HE) histology from teratoma tissue in nude mice kidney capsule grown for 6 weeks, showing striated muscle (mu) and a vessel (ve) of mesodermal origin, endodermal-epithelia (en-ep) of intestinal character, and ectodermal epithelia of peridermal (p-ep) character. Scale bars: 100 µm.