Motoneuron Differentiation of Induced Pluripotent Stem Cells from SOD1G93A Mice - Figure 2

<p>Immunostaining shows that the established iPS cell line (ALS-iPS-1) was positive for Oct4 (A) and SSEA-1 (B). (C) The expression patterns of pluripotent genes in iPS clones, E14 cells, and fibroblasts. The results revealed that all analyzed iPS clones induced expression from the endogenous Oct4, Sox2, and Nanog loci, and none of these genes were expressed in the original TTF fibroblasts. (D–F) Teratoma derived from ALS-iPS-1 cells contained cells belonging to all three germ layers, including endoderm-derived glandular (D), mesoderm-derived cartilage tissue (E), and ectoderm-derived neural tubes (F). Karyotype analyses demonstrated that ALS-iPS-1 clones showed a normal karyotype (G). Scale bar: 100 µm in A and B; 250 µm in D, E, and F.