Aberrant methylation targets a minimally overlapping set of CpGs in IDH-mut and MLLr AMLs.

<p>(A) Venn diagram representing differentially methylated CpGs identified for IDH-mut and MLLr AMLs from amongst the 574,178 CpGs adequately represented (>10× coverage) across all six samples. Most DMCs are unique to either AML subtype, with minimal amount of events occurring at common sites across IDH-mut and MLLr. (B) Horizontal barplot comparing the methylation status of CpG sites at DMCs in IDH-mut AML (top) and MLLr AML (bottom). Magenta depicts >25% hypermethylation relative to NBM, green represents >25% hypomethylation and gray represents no differential methylation. Most DMCs are non-overlapping between the two subtypes of AML and display opposite changes in methylation. However, amongst the smaller set of DMCs that do overlap between the two AML subtypes, the vast majority (76.6%) are concordantly changed, with a clear predominance for aberrrant hypermethylation of those sites (79%).