5′-RLM-RACE verification of predicted miRNA targets.

<p>Each top strand depicts a miRNA complementary site (targets), and each bottom strand depicts the miRNA. miR159 and miR319 shares the target sequence. Watson-Crick pairing (vertical dashes) and G:U wobble pairing (circles) are indicated. Arrows indicate the 5′termini of mRNA fragments isolated from roots of the maize seedlings, as identified by cloned 5′-RACE products, with the frequency of clones shown. Only cloned sequences that matched the correct target gene and had 5′ ends within a 100 nt window centered on the miRNA complementary site are counted.