<p>The cleavage sites were determined by 5′ RLM-RACE. The nucleotide positions of the miRNA complementary sites of unigenes are indicated. The unigene sequence of each complementary site from 5′ to 3′ and the cloned miRNA sequence from 3′ to 5′ are shown. Watson-Crick pairing (vertical dashes) and G:U wobble pairing (circles) are indicated. Vertical arrows indicate the 5′ termini of miRNA-guided cleavage products, as identified by 5′ RLM-RACE, with the frequency of clones shown.</p
<p>The target of miR166 (TC04758) encodes a transcription factor belonging to class III of the HD-ZI...
<p>The cleavage sites and the number of sequenced clones corresponding to each site are indicated by...
<p>(A) T-plot (top) and miRNA: mRNA alignments (bottom) for two category I targets, Unigene11776_All...
<p>The cleavage sites of two selected targets in two miRNA as identified by 5′ RACE analysis. For ea...
<p>Cleavage sites were determined by the modified 5’RNA ligase-mediated RACE. Heavy black lines repr...
<p>Each top strand depicts a miRNA complementary site (targets), and each bottom strand depicts the ...
<p>(A) Gel electrophoresis of 5′ RACE PCR products of target genes for miRNAs. The red triangles ind...
<p>Agarose gel image of 5′ RACE products (A) and the target mRNA cleavage sites (B). The targeted mR...
<p>Targets of the <i>B. juncea</i> miR156 (Bju-miR156), miR160 (Bju-miR160) and miR164 (Bju-miR164) ...
<p>The vertical lines represent the nucleotides that base-pair with GRMZM2G028054_T01 and dots show ...
<p>(A) miR173-mediated cleavage of miR173ts_CHS transcripts. The cleavage position and the number of...
<p>Arrows indicate the 5’ ends of mRNA fragments, as identified by cloned 5’ RLM-RACE products, with...
<p>Nucleotide sequence conservation between the 3′ UTRs of human and the closest mouse or rat orthol...
<p>The arrows indicate the cleavage sites and the numbers show the frequency of clones sequenced.</p
5' RLM RACE was employed so as to map the cleavage sites. The coding sequence of the target gene was...
<p>The target of miR166 (TC04758) encodes a transcription factor belonging to class III of the HD-ZI...
<p>The cleavage sites and the number of sequenced clones corresponding to each site are indicated by...
<p>(A) T-plot (top) and miRNA: mRNA alignments (bottom) for two category I targets, Unigene11776_All...
<p>The cleavage sites of two selected targets in two miRNA as identified by 5′ RACE analysis. For ea...
<p>Cleavage sites were determined by the modified 5’RNA ligase-mediated RACE. Heavy black lines repr...
<p>Each top strand depicts a miRNA complementary site (targets), and each bottom strand depicts the ...
<p>(A) Gel electrophoresis of 5′ RACE PCR products of target genes for miRNAs. The red triangles ind...
<p>Agarose gel image of 5′ RACE products (A) and the target mRNA cleavage sites (B). The targeted mR...
<p>Targets of the <i>B. juncea</i> miR156 (Bju-miR156), miR160 (Bju-miR160) and miR164 (Bju-miR164) ...
<p>The vertical lines represent the nucleotides that base-pair with GRMZM2G028054_T01 and dots show ...
<p>(A) miR173-mediated cleavage of miR173ts_CHS transcripts. The cleavage position and the number of...
<p>Arrows indicate the 5’ ends of mRNA fragments, as identified by cloned 5’ RLM-RACE products, with...
<p>Nucleotide sequence conservation between the 3′ UTRs of human and the closest mouse or rat orthol...
<p>The arrows indicate the cleavage sites and the numbers show the frequency of clones sequenced.</p
5' RLM RACE was employed so as to map the cleavage sites. The coding sequence of the target gene was...
<p>The target of miR166 (TC04758) encodes a transcription factor belonging to class III of the HD-ZI...
<p>The cleavage sites and the number of sequenced clones corresponding to each site are indicated by...
<p>(A) T-plot (top) and miRNA: mRNA alignments (bottom) for two category I targets, Unigene11776_All...