Distances between lid α5 and α10 at different sets of simulations.

<p>Inter helical distance of three different simulations of CALB at 5°C for 30 ns. First experiment (blue), second experiment (red) and third experiment (green) (A). Helical distance of long–term simulation of CALB at 5°C (blue), 35°C (red) and 50°C (green) for 60 ns. The structure closed and re-opened at 23 and 31 ns of simulation sequentially at 5°C (B).