Isothermal titration calorimetry studies of the interaction of the BACHEMP-Cons peptide with CaCl₂.

<p>(A) Upper panel: raw data for the titration of 50 µM peptide with 3.2 µl aliquots of 5 mM CaCl₂. Experiments were conducted in polybuffer at the pH values indicated. Lower panel: Integrated, dilution-corrected and concentration-normalized raw data. Data were fitted with the “One binding site model” of the MicroCal (Northampton, MA) version of ORIGIN. pH 6.0 (○), pH 7.0 (□), pH 8.0 (Δ). The derived thermodynamic parameters are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040698#pone-0040698-t004" target="_blank">Table 4</a>. (B) Dependence of <i>K</i>_D on the pH. Experiments were conducted in polybuffer which was adjusted to the pH indicated by the addition of concentrated HCl or NaOH. Shown are means and standard errors derived from three individual experiments.