Isotermal titration calorimetry analysis of the interaction of μ4 with the APP sorting signal.

<p>Isothermal titration calorimetry of the APP ENPTYKFFEQ peptide with recombinant C-terminal domain of wild-type μ4 (<b>A</b>), μ4-D190A (<b>B</b>), or μ4-R283D (<b>C</b>). The stoichiometry (N) and <i>K_d</i> for the interaction of the ENPTYKFFEQ peptide with either μ4-WT or μ4-D190A are expressed as the mean ± SEM (n = 3). Because the interaction of the ENPTYKFFEQ peptide with μ4-R283D is undetectable the stoichiometry and <i>K_d</i> were not determined (N/D).