Fluorescence intensity of CD4 T cells following <i>in vitro</i> stimulation with the antigen BCG.

<p>(A) Fluorescence intensity of single-(yellow), double-(blue), or triple-(red) cytokine-producing CD4 T cells in a representative child 10 weeks after BCG immunisation. Events are plotted on a logarithmic scale. Cells in the top right hand corner are those producing the greatest amount of both cytokines. (B, C) Median fluorescence intensity (MFI) for IFN-γ-, IL-2- and TNF-α-producing CD4 T cell subpopulations following <i>in vitro</i> stimulation with the antigen BCG for (B) children (n = 13) and (C) adults (n = 13) 10 weeks after BCG immunisation. White bars show total cytokine-producing populations. Whiskers indicate IQR. Bars with * are based on one value only. Overall comparison was done using a Kruskal-Wallis test and comparisons of two populations were done using a Mann-Whitney test. The numbers above the horizontal bars represent the p-values for comparisons. ns = not significant (p>0.05).