Intracellular localization of the Flag-Tax proteins.

<p>293 T cells were transduced by Lenti-IRES-GFP or Lenti-Flag-Tax lentiviruses for 72 h. Cells were fixed on Lab-tek slides and stained with the mouse monoclonal antibody anti-Flag-M2 (Sigma) followed by a goat anti-mouse CY3-conjugated secondary antibody (Amersham Biosciences). Nucleic acids were stained with DAPI-containing mounting medium (Vectashield, Vector Laboratories). Cells were vizualized using with a Zeiss Axioplan 2 imaging microscope, X40, and the SimplePCI software (Hamamatsu).