Transcriptional activity of the Flag-Tax proteins on the HTLV-1 promoter or on a synthetic NF-κB

<p><b>promoter.</b> (A, B): 293 T cells were transduced by Lenti-Flag-Tax lentiviruses for 48 h. Cells were then transfected with (A) 500 ng of HTLV-1-LTR-Luc or (B) 500 ng of NF-κB-Luc reporter plasmid. Transfection results were normalized to Renilla activity by transfecting 10 ng of phRG-TK (Promega), and DNA quantities were adjusted with vector control. The results presented in (A) and (B) are the average of at least 3 independent experiments. Tables represent the fold-activation of the different Flag-Tax proteins over the control. (C, D) Western blot analyses were performed on 70 µg of cellular extracts from 293 T cells transduced in A and B. Membranes were probed with anti-Flag-M2 or anti-β-actin antibody (Sigma).