PMN release IL-8 upon stimulation with aPL and LPS.

<p>PMN (2×10⁵ cells per well) were incubated with medium, aPL or hIgG (10 µg/ml) in the presence or absence of LPS. Supernatants were harvested and an IL-8 specific ELISA was performed after A) 2 hours or B) 6 hours of incubation (37°C). Data shown are from one representative experiment out of 3 independent with 2 replicates per group. * Indicates significant difference of LPS-stimulated groups compared to corresponding hIgG controls (p<0.05 in a Tukey’s multiple comparison test).