Autolysis of <i>atl</i> mutants.

<p>Triton X-100 induced autolysis of mid-exponential phase cultures grown in TSB with 1 M glucose at a 1∶10 media to flask volume ratio. A) comparison of UAMS-1 (solid diamonds), Δ<i>atl</i> mutant (empty circles), Δ<i>atl_AM</i> mutant (empty triangles), and Δ<i>atl_GL</i> mutant (empty squares). B) UAMS-1 (solid diamonds), Δ<i>atl</i> mutant (empty circles), and mutant with <i>atl</i> complement plasmid pJB141 (solid circles). C) UAMS-1 (solid diamonds), Δ<i>atl_AM</i> mutant (empty triangles), and mutant with <i>atl_AM</i> complement plasmid pJB111 (solid triangles). D) UAMS-1 (solid diamonds), Δ<i>atl_GL</i> mutant (empty squares), and mutant with <i>atl_GL</i> complement plasmid pJB135 (solid squares). B–D) UAMS-1 and mutants contain vector alone plasmid pJB128. Data represent the mean (n = 3 for panel A, n = 2 for B–D) with standard error (bars are smaller than symbols).