Effect of <i>atl</i> point mutations on murein hydrolase activity.

<p>A) Triton X-100 induced autolysis of mid-exponential phase cultures grown in TSB with 1 M NaCl at a 1∶10 media to flask volume ratio. UAMS-1 pJB128 (solid diamonds), Δ<i>atl_AM</i> mutant pJB128 (empty triangles), mutant with <i>atl_AM</i> complement plasmid pJB111 (solid triangles), mutant with <i>atl_AMH263A</i> complement plasmid pJB122 (empty square), and mutant with <i>atl_AMH380A</i> complement plasmid pJB123 (empty circles). B) Quantitative murein hydrolase activity of culture supernatants on <i>Micrococcus</i> cells. UAMS-1(pJB128) (solid diamonds), Δ<i>atl_GL</i> mutant KB5001 (empty diamonds), mutant with <i>atl_GL</i> complement plasmid pJB135 (solid squares), and mutant with <i>atl_GLE1129A</i> complement plasmid pJB142 (empty squares). Data represent the mean (n = 2) with standard error.