The expression of h PARP-1 <i>in vivo</i> increases the luciferase enzyme activities assayed in Del4 reporter plasmid transfected PARP −/− MEF cells.

<p>Logarithmically growing PARP −/− MEF cells were transfected with Del-4 plasmid together with the pcDNA3.1-<i>beta-galactosidase</i> expressing plasmid and in the absence or in the presence of h PARP-1 expression (pcDNA3.1-<i>parp-1</i> plasmid). After two days of incubation cells were splitted into six-well plates and grown for a day further. Than cells were harvested, lysed and their luciferase and beta-galactosidase enzyme activities were determined. The luciferase enzyme activities were normalized for beta-galactosidase activities and are shown in the figure. Asterisks show significant difference in the reporter enzyme activities between the two pools of sample (p<0.05).