Luciferase measurements of MS-KIF18A promoter activity.

<p>(A) Schematic illustration of MS-KIF18A promoter-luciferase reporter constructs. MCF-7 cells transfection with MS-KIF18A promoter cloned in luciferase reporter plasmid (pGLuc-K) or promoter less pGL3-basic along with β-galactosidase vector. (B) Cells treated (black bars) or not (white bars) with 17βE₂ (10⁻⁸ M) for 24 h; (C) 17βE₂ (10⁻⁸ M) or/and ICI-182,780 (10⁻⁷ M) were added to the cultures for 1 h (white bars) or 24 h (gray bars). Promoter activities are expressed as luciferase values normalized for β-galactosidase levels. A value of 100% was given to the basal promoter activity elicited by the pGLuc-K construct in the absence of any treatment. Results are mean±SD of 3 independent experiments, performed in duplicates.