Generation of conditional <i>mir-181</i> alleles in mice.

<p>(A) Schematics of putative primary miR-181 transcripts. (B) Alignment of mouse miR-181 family mature miRNAs. (C) Predicted stem-loop structures of miR-181 family miRNAs. (D-F) PCR confirmation of the deletion of neo cassette and (D) <i>mir-181ab1</i>, (E) <i>mir-181ab2</i> and (F) <i>mir-181c/d</i>. (G) RT-qPCR analyses of primary miR-181 expression in the thymus (blue) and spleen (red) of <i>mir-181</i> knockout mice. (H) Northern blot analyses to confirm loss of miR-181a and miR-181b expression in the thymus upon the loss of <i>mir-181ab1</i> alleles. (I) Mature miR-181a and miR-181b expression in DP cells from WT, <i>mir-181ab1</i>^−/− and <i>mir-181ab2</i>^−/− mice determined by quantitative miRNA PCR analyses (n = 2, *, <i>p</i><0.05, Student's <i>t</i>-test).