The differential effects of loss of individual <i>mir-181</i> family genes on ICN1-induced T-ALL.

<p>(A and B) Copy numbers of mature miR-181a, miR-181b, miR181c and miR-181d in ICN1-infected DP cells from (A) ICN1:<i>181ab1^+/+</i> and (B) ICN1:<i>181ab1^−/−</i> recipient mice at 2, 4 and 6 weeks post-transplantation determined by standard curve-based miRNA qPCR analyses. (C and D) Kaplan-Meier survival curves indicate the percentage of mice (20 mice/group) surviving at different time points after reconstituting with (C) ICN1-infected <i>mir-181ab1/ab2^−/−</i> (<i>p</i><0.001) or (D) ICN1-infected <i>mir-181ab1/cd^−/−</i> BM cells (<i>p</i>>0.05). (E and F) Effects of loss of <i>mir-181</i> family genes on the percentage of total ICN1-infected cells (all GFP⁺ cells) in peripheral blood at different time points after reconstitution (**, <i>p</i><0.01).