Activation of JNK was involved in TAp73α-mediated apoptosis induced by cisplatin.

<p>(A) TAp73α-overexpressed cells (SKOV3 C8 and OVCA433 C1) were treated with 20 μM SP600125 for different periods of time (indicated). The phosphorylation levels of JNK and c-Jun were measured. (B and C) TAp73α-overexpressed cells (SKOV3 C8 and OVCA433 C1) and the empty vector controls (V) were treated with 20 μM SP600125 or DMSO and then with cisplatin. The cell apoptosis were assessed by TUNEL assay (error bars indicated mean ± SD from three independent experiments; *: p<0.05) and the cleavage of PARP analysis. Inhibition of JNK attenuated TAp73α-mediated apoptosis in response to cisplatin. (D) TAp73α-overexpressed cells (SKOV3 C8 and OVCA433 C1) were treated with JNK siRNAs (Si-JNK) or the scrambled control siRNA (Control). The activations of JNK and c-Jun were absent upon cisplatin treatment, and associated with markedly reduced cell apoptosis (E and F).