GFAPα transfected cells stained with various GFAP antibodies.

<p>All panels show SW13/cl.2 cells transfected with full length msGfap-α, stained with GFAP monoclonal antibody to detect successfully transfected cells and double stained with the different polyclonals. (<b>A–A’</b>) GFAPpan and (<b>B–B’</b>) GFAPc-term antisera are able to detect GFAPα composed IF networks, whereas (<b>C–C’</b>) msGFAPδ, (<b>D–D’</b>) msGFAPκ, and (<b>E–E’</b>) msGFAP+1 display no reactivity against the canonical GFAPα. Panels <b>A–E</b> show DAPI staining, a fluorescent stain that binds to DNA.