GFAP-SB11 transgenics express functional transposase in a subset of GFAP⁺ cells.

<p>Two lines (A and B) were established and used for these experiments. SB = SB transposase, T2 = T2/onc, LV = lateral ventricle. A) Immunofluorescence for GFAP (red) and SB (green). Nuclei are stained with DAPI. Arrows indicate examples of SB⁺ GFAP⁺ cells while asterisks indicate examples of SB⁻ GFAP⁺ cells. Scale bars are 20 µm. B) PCR based excision assay showing that transposons have mobilized in the brains of SB⁺T2⁺ but not SB⁻T2⁺ or SB⁺T2⁻ mice from each line. A control PCR demonstrates that genomic DNA is present for all samples.