Altered arterial contractility to a vasoconstrictor (Ang II) in ISO-CAs.

<p>A. Representative trace of Ang II dose response vascular contraction in control and ISO-CAs. B. Line graph of contractile dose response to Ang II in control and ISO-CAs. (n = 4, mean ± SEM, two-way ANOVA test, *P<0.05). C. Ang II–induced intracellular calcium peak changes in control and ISO-CAs. D. Quantitative group data of calcium release, Ang II-induced [Ca²⁺]_i change calculated by Δ(<i>peak</i>340/380-<i>basal</i>340/380) of each group. (n = 4, mean ± SEM, Student's t-test,*P<0.05). E. F. Intracellular calcium changes were measured by confocal microscopy in basal and after 20 min of Ang II treatment. ISO-CAs has lower response to Ang II-induced intracellular calcium increase (52.35±1.23 vs. 25.76±2.12, n = 4, Student's t-test,*P<0.05). G. Ang II-induced ROS productions were measured by confocal microscopy in basal (0 min) and after 10 and 20 min of Ang II treatment. ISO-CAs has lower response to Ang II-induced ROS production.