Effect of ANG II on intracellular calcium response in renal VSM cells.

<p>The upper panel presents a representative tracing of a [Ca²⁺]_i response of a single renal microvessel VSM cell to ANG II (10^-7 M) bathed in PSS before and after addition of ionomycin (10^-6 M). ANG II elicited a rise in [Ca²⁺]_i in renal VSM cells under control conditions. Addition of ionomycin raised baseline [Ca²⁺]_i and under these conditions ANG II had no additional effect on [ Ca²⁺]_i. The lower left panel summarizes the effects of on peak and plateau [Ca²⁺]_i responses to ANG II (10^-7 M) in the renal VSM cells. The right panel presents the [Ca²⁺]_i response to ANG II after addition of ionomycin. * indicates a significant rise in [Ca²⁺]_i over baseline. Mean values <u>+</u> SE recorded from 45-70 cells (5-10 cells per experiment) isolated from 9 different renal VSM cell isolation are presented.