Expression analysis of transcript variants of <i>DDB1</i>.

<p>(A) Schematic diagram of <i>DDB1</i> gene splicing pattern. Introns (I) and exons (E) are marked by lowercase and uppercase letters, respectively. Numbers above the letter and arrows indicate the position of the nucleotide in the genome sequence and the site of alternative splicing, respectively. The extra 15 bp nucleotides of <i>DDB1⁺¹⁵</i> transcript are shown in grey. (B) The percentage of two <i>DDB1</i> transcripts in different tissues. SD: seedlings; R: roots; SM: stems; L: leaves; F: flowers; IG1: immature green fruits at 7-DPA; IG2: immature green fruits at 14-DPA; MG: mature green fruits at 35-DPA. The light gray and black bars indicate the expression percentage of the <i>DDB1⁺¹⁵</i> and <i>DDB1^F</i> transcripts, respectively. Data presented are the means of three technical replicates. Similar results were obtained in at least two independent experiments. (C) Identification of the percentage of two <i>DDB1</i> transcripts in seedlings under hormones or stress treatment. Total RNAs were extracted from 10-day-old seedlings with application of abscisic acid (ABA), auxin (indole acetic acid; IAA), cytokinin (6-benzylaminopurine, BA), gibberellins (GA) and mannitol. 10-day-old seedlings without any hormone and stress treatment were used as control. The light gray and black bars indicate the expression percentage of the <i>DDB1⁺¹⁵</i> and <i>DDB1^F</i> transcripts, respectively.