<i>DDB1</i> mRNA level analysis and phenotypes in transgenic plants.

<p>(A) Real-time PCR analysis of total <i>DDB1</i> mRNA level in WT Ailsa Craig (<i>WT</i>), <i>35S::DDB1⁺¹⁵</i> and <i>35S::DDB1^F</i> T₀ transgenic lines. a–j and a–i stand for 10 and 9 independent transgenic lines for <i>35S::DDB1⁺¹⁵</i> and <i>35S::DDB1^F</i> constructs, respectively. Each bar represents three repetitions from each RNA sample (derived from pools of at least three fruits per plant). Error bars representing standard errors are shown in each case. (B) Representative red ripe fruits from field-grown plants of WT Ailsa Craig (<i>WT</i>) and 3 independent T₀ transgenic lines (a, b and c) containing <i>35S::DDB1⁺¹⁵</i> or <i>35S::DDB1^F</i>. Bar = 1 cm. (C) Comparison of red ripe fruit weight from WT Ailsa Craig (<i>WT</i>) and 3 independent T₀ transgenic lines (a, b and c) containing <i>35S::DDB1⁺¹⁵</i> or <i>35S::DDB1^F</i>. Mean values from 10 fruits from each line and standard errors are shown. Statistical analysis was performed using Student's <i>t</i>-test. (*P<0.05, **P<0.01). (D) Mature green fruits from WT Ailsa Craig (<i>WT</i>), <i>35S::DDB1⁺¹⁵</i> and <i>35S::DDB1^F</i> T₁ transgenic lines. (E) Comparison of red ripe fruit weight of T₁, T₂ and T₃ nullizygous <i>35S::DDB1^F</i> plants segregated out from 3 T₀ transgenic lines (lines a, b and c). Mean values from 10 fruits from each line and standard errors are shown. Error bars representing standard errors are shown in each case. p and n represent plants with or without transgene, respectively.