MES+HS suppresses the expressions of pro-inflammatory cytokines in kidneys of Alport mice in vivo.

<p>(<i>a–j</i>) Forty-eight hours after the last treatment, total RNA was isolated from kidneys (<i>a</i>–<i>f</i>) or glomeruli (<i>g</i>–<i>j</i>) of Alport mice sham-treated (CON) or treated with MES+HS for 8 weeks. Q-PCR analysis was performed to assess the expression of the indicated cytokines (<i>a</i>–<i>e, g</i>–<i>j</i>) and renal injury marker NGAL (<i>f</i>). Values are the mean ± S.E. (n = 6). *<i>P</i><0.05, *** <i>P</i><0.001 assessed by unpaired <i>t</i>-test. n.s., not significant.