Renal inflammation and injury in D₂−/− mice.

<p>Masson stained sections of D₂+/+ mouse kidney (<b>A</b> and <b>B</b>) and D₂−/− mouse kidney (<b>C</b> and <b>D</b>). H-E stained sections of D₂+/+ mouse kidney (<b>E</b>) and D₂−/− mouse kidney (<b>F</b>). G: glomerulus. PCT: proximal convoluted tubule. Proteinaceous casts are marked with arrows (<b>F</b>). Sections from 3 mouse kidneys per group were studied. <b>G</b> and <b>H</b>: Inflammatory cell infiltration. Kidney sections from D₂+/+ (<b>G</b>) and D₂−/− (<b>H</b>) mice were immunostained for the presence of macrophages and monocytes (arrows). The number of positive cells in 10 randomly selected fields was greater in D2−/− (68±3) than in D2+/+ (15±1, P<0.01) mice. Sections from 3 mouse kidneys per group were studied. <b>I</b>. Renal cortical expression of Col 1α1 mRNA determined by qRT-PCR. Results were corrected for expression of GAPDH mRNA and expressed as fold change in comparison to their expression in D₂+/+ mice. *P<0.05 vs D₂+/+; n = 5/group. <b>J</b>. Urinary microalbuminuria. Urine samples were collected for 24 h from mice in metabolic cages. Albumin was measured by ELISA. *P<0.04 vs. D₂+/+; n = 5/group. Magnification: A and C: 100X; B, D, G and H: 400X; E-F: 200X.