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T-cell proliferation in response to mitogens and antigens, as assessed by thymidine incorporation<sup>A</sup>.

Amandine Crequer (140157)
Capucine Picard (64703)
Etienne Patin (107311)
Aurelia D’Amico (140162)
Avinash Abhyankar (140166)
Martine Munzer (140170)
Marianne Debré (78202)
Shen-Ying Zhang (140174)
Geneviève de Saint-Basile (140177)
Alain Fischer (5250)
Laurent Abel (69833)
Gérard Orth (140180)
Jean-Laurent Casanova (64712)
Emmanuelle Jouanguy (64694)

Publication date

August 2012

DOI

10.1371/journal.pone.0044010.t002

Abstract

A<p>3H-TdR incorporation in cpm/10<sup>3</sup>.</p>B<p>normal ranges from internal laboratory controls.</p

Extracted data

Related items

Measurement of T-lymphocyte responses in whole blood cultures using newly synthesized DNA

P. R. Sottong
J. A. Rosebrock
J. A. Britz
T. R. Kramer

January 2000

The proliferative response is most frequently determined by estimating the amount of [3H]thymidine i...

Allogeneic mixed lymphocyte reactions.

Hui-feng Yang (300559)
Ze-hua Zhang (5651692)
Liang-bi Xiang (300560)
Kang-lai Tang (300561)
Fei Luo (289384)
Chun-yu Liu (1865890)
Jian-bo Zhou (300562)
Jin-qing Li (300563)
Jian-zhong Xu (5651695)

February 2013

<p>The allostimulatory capacities of the −/+25(OH)D<sub>3</sub>, 1,25(OH)2D<sub>3</sub>(10 pM) and 1...

TIM-3 antibodies do not affect human T cell activation.

Judith Leitner (392966)
Armin Rieger (322069)
Winfried F. Pickl (392967)
Gerhard Zlabinger (392968)
Katharina Grabmeier-Pfistershammer (322059)
Peter Steinberger (392969)

March 2013

<p>CD4 T cells were stimulated with plate-bound antibodies to CD3 and CD28 immobilized at the indica...

Results showing the uptake of thymidine by actively growing cells in respective experiments of primary and secondary stimulation.

Ravi Kumar Gupta (349589)
Sanjay Chhibber (283732)
Kusum Harjai (349590)

February 2013

<p>Splenocytes were incubated with different concentrations of synthetic AHLs (OdDHL and BHL) at 37°...

Mature DC cannot substitute for immature DC at either stimulation time points.

Thomas G. Berger (371146)
Hendrik Schulze-Koops (26044)
Michaela Schäfer (371147)
Ester Müller (371148)
Manfred B. Lutz (114898)

February 2013

<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...

Allogeneic CD4<sup>+</sup> T cell proliferation induced by Ctrl-MoDC, PTEC-MoDC and PTEC-MoDC supplemented with IDO inhibitor 1-MT represented as mean values for five donors (bar graph) and five individual donors (line graph) at a 1:8 MoDC:T cell ratio.

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July 2015

<p>Proliferation was measured by <sup>3</sup>[H]-thymidine incorporation and expressed as counts per...

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Luuk B. Hilbrands (87735)
Hans J. P. M. Koenen (87732)
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February 2013

<p>Treg and Tconv were expanded according the schedule in <a href="http://www.plosone.org/article/in...

Deficient proliferation of Mst1<sup>−/−</sup> T and B cells in vitro.

Konstantin V. Salojin (567844)
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Wei Chun Chang (43479)
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Jeannette Crisostomo (567846)
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Julia Zhou (567850)
Amr Nouraldeen (567851)
Theodore C. Jessop (567852)
Jeffrey T. Bagdanoff (567853)
David J. Augeri (567854)
Robert Read (375290)
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Alan Main (567856)
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Tamas Oravecz (238781)

May 2014

<p>Single cell suspensions of splenic T and B lymphocytes were stimulated with various T and B cell-...

Allogeneic CD4<sup>+</sup> T cell proliferation induced by Ctrl- and PTEC-MoDC from CD and CI cultures in three individual donors (donor 1, donor 2 and donor 3) at 1:8 MoDC:T cell ratio.

Sandeep Sampangi (515426)
Andrew J. Kassianos (515422)
Xiangju Wang (515421)
Kenneth W. Beagley (157699)
Travis Klein (390441)
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July 2015

<p>Proliferation was measured by <sup>3</sup>[H]-thymidine incorporation and expressed as counts per...

Proliferative response of PBMC to mitogens.

Pramod N. Nehete (221188)
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Bharti P. Nehete (429902)
Guojun Yang (221194)
Julio C. Ruiz (489263)
Lawrence Williams (489264)
Christian R. Abee (489265)
K. Jagannadha Sastry (221197)

November 2013

<p>PBMC isolated from the blood samples of the squirrel monkeys were used for determining proliferat...

1-D-MT reduces T cell proliferation in cocultures of SKOV-3 cells with mixed leukocyte reactions.

Christiane A. Opitz (351069)
Ulrike M. Litzenburger (126790)
Uta Opitz (351071)
Felix Sahm (126791)
Katharina Ochs (351073)
Christian Lutz (351075)
Wolfgang Wick (126794)
Michael Platten (34815)

February 2013

<p>(<b>A</b>) Alloreactive T cell proliferation after addition of 25 µM kyn to mixed leukocyte react...

Proliferative responses of MLN (A) and spleen (B) cells from uninfected and infected hamsters to purified rTsCRT and TsCE.

Fela Mendlovic (713298)
Mayra Cruz-Rivera (713299)
Guillermina Ávila (713300)
Gilberto Vaughan (363289)
Ana Flisser (331998)

March 2015

<p>White bars represent data from cells incubated in RPMI alone, grey bars from cells incubated with...

Immunological assay.

Damien Restagno (3092421)
Fabienne Venet (432807)
Christian Paquet (827905)
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August 2016

<p>Spleens were harvested at D5 and splenocytes were rapidly isolated in order to determine T cells ...

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Thomas G. Berger (371146)
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Michaela Schäfer (371147)
Ester Müller (371148)
Manfred B. Lutz (114898)

February 2013

<p>DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimu...

Proliferative capacity of expanded Treg.

Jorieke H. Peters (270898)
Luuk B. Hilbrands (87735)
Hans J. P. M. Koenen (87732)
Irma Joosten (27492)

February 2013

<p>Treg and Tconv were expanded according the schedule in <a href="http://www.plosone.org/article/in...

Measurement of T-lymphocyte responses in whole blood cultures using newly synthesized DNA

P. R. Sottong
J. A. Rosebrock
J. A. Britz
T. R. Kramer

January 2000

The proliferative response is most frequently determined by estimating the amount of [3H]thymidine i...

Allogeneic mixed lymphocyte reactions.

Hui-feng Yang (300559)
Ze-hua Zhang (5651692)
Liang-bi Xiang (300560)
Kang-lai Tang (300561)
Fei Luo (289384)
Chun-yu Liu (1865890)
Jian-bo Zhou (300562)
Jin-qing Li (300563)
Jian-zhong Xu (5651695)

February 2013

<p>The allostimulatory capacities of the −/+25(OH)D<sub>3</sub>, 1,25(OH)2D<sub>3</sub>(10 pM) and 1...

TIM-3 antibodies do not affect human T cell activation.

Judith Leitner (392966)
Armin Rieger (322069)
Winfried F. Pickl (392967)
Gerhard Zlabinger (392968)
Katharina Grabmeier-Pfistershammer (322059)
Peter Steinberger (392969)

March 2013

<p>CD4 T cells were stimulated with plate-bound antibodies to CD3 and CD28 immobilized at the indica...

Results showing the uptake of thymidine by actively growing cells in respective experiments of primary and secondary stimulation.

Ravi Kumar Gupta (349589)
Sanjay Chhibber (283732)
Kusum Harjai (349590)

February 2013

<p>Splenocytes were incubated with different concentrations of synthetic AHLs (OdDHL and BHL) at 37°...

Mature DC cannot substitute for immature DC at either stimulation time points.

Thomas G. Berger (371146)
Hendrik Schulze-Koops (26044)
Michaela Schäfer (371147)
Ester Müller (371148)
Manfred B. Lutz (114898)

February 2013

<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...

Allogeneic CD4<sup>+</sup> T cell proliferation induced by Ctrl-MoDC, PTEC-MoDC and PTEC-MoDC supplemented with IDO inhibitor 1-MT represented as mean values for five donors (bar graph) and five individual donors (line graph) at a 1:8 MoDC:T cell ratio.

Sandeep Sampangi (515426)
Andrew J. Kassianos (515422)
Xiangju Wang (515421)
Kenneth W. Beagley (157699)
Travis Klein (390441)
Sadia Afrin (555214)
Helen Healy (515429)
Ray Wilkinson (515420)

July 2015

<p>Proliferation was measured by <sup>3</sup>[H]-thymidine incorporation and expressed as counts per...

Suppressive capacity of expanded Treg in [<sup>3</sup>H]Thymidine incorporation suppression assays.

Jorieke H. Peters (270898)
Luuk B. Hilbrands (87735)
Hans J. P. M. Koenen (87732)
Irma Joosten (27492)

February 2013

<p>Treg and Tconv were expanded according the schedule in <a href="http://www.plosone.org/article/in...

Deficient proliferation of Mst1<sup>−/−</sup> T and B cells in vitro.

Konstantin V. Salojin (567844)
Brian D. Hamman (567845)
Wei Chun Chang (43479)
Kanchan G. Jhaver (238746)
Amin Al-Shami (238743)
Jeannette Crisostomo (567846)
Carrie Wilkins (238753)
Ann Marie Digeorge-Foushee (567847)
Jason Allen (329915)
Nita Patel (567848)
Suma Gopinathan (567849)
Julia Zhou (567850)
Amr Nouraldeen (567851)
Theodore C. Jessop (567852)
Jeffrey T. Bagdanoff (567853)
David J. Augeri (567854)
Robert Read (375290)
Peter Vogel (238749)
Jonathan Swaffield (375294)
Alan Wilson (230195)
Kenneth A. Platt (133502)
Kenneth G. Carson (567855)
Alan Main (567856)
Brian P. Zambrowicz (304232)
Tamas Oravecz (238781)

May 2014

<p>Single cell suspensions of splenic T and B lymphocytes were stimulated with various T and B cell-...

Allogeneic CD4<sup>+</sup> T cell proliferation induced by Ctrl- and PTEC-MoDC from CD and CI cultures in three individual donors (donor 1, donor 2 and donor 3) at 1:8 MoDC:T cell ratio.

Sandeep Sampangi (515426)
Andrew J. Kassianos (515422)
Xiangju Wang (515421)
Kenneth W. Beagley (157699)
Travis Klein (390441)
Sadia Afrin (555214)
Helen Healy (515429)
Ray Wilkinson (515420)

July 2015

<p>Proliferation was measured by <sup>3</sup>[H]-thymidine incorporation and expressed as counts per...

Proliferative response of PBMC to mitogens.

Pramod N. Nehete (221188)
Patrick W. Hanley (429904)
Bharti P. Nehete (429902)
Guojun Yang (221194)
Julio C. Ruiz (489263)
Lawrence Williams (489264)
Christian R. Abee (489265)
K. Jagannadha Sastry (221197)

November 2013

<p>PBMC isolated from the blood samples of the squirrel monkeys were used for determining proliferat...

1-D-MT reduces T cell proliferation in cocultures of SKOV-3 cells with mixed leukocyte reactions.

Christiane A. Opitz (351069)
Ulrike M. Litzenburger (126790)
Uta Opitz (351071)
Felix Sahm (126791)
Katharina Ochs (351073)
Christian Lutz (351075)
Wolfgang Wick (126794)
Michael Platten (34815)

February 2013

<p>(<b>A</b>) Alloreactive T cell proliferation after addition of 25 µM kyn to mixed leukocyte react...

Proliferative responses of MLN (A) and spleen (B) cells from uninfected and infected hamsters to purified rTsCRT and TsCE.

Fela Mendlovic (713298)
Mayra Cruz-Rivera (713299)
Guillermina Ávila (713300)
Gilberto Vaughan (363289)
Ana Flisser (331998)

March 2015

<p>White bars represent data from cells incubated in RPMI alone, grey bars from cells incubated with...

Immunological assay.

Damien Restagno (3092421)
Fabienne Venet (432807)
Christian Paquet (827905)
Ludovic Freyburger (3092424)
Bernard Allaouchiche (131964)
Guillaume Monneret (329582)
Jeanne-Marie Bonnet (466631)
Vanessa Louzier (827904)

August 2016

<p>Spleens were harvested at D5 and splenocytes were rapidly isolated in order to determine T cells ...

LowGM-DC remain functionally immature after maturation.

Thomas G. Berger (371146)
Hendrik Schulze-Koops (26044)
Michaela Schäfer (371147)
Ester Müller (371148)
Manfred B. Lutz (114898)

February 2013

<p>DC were generated under low-GM (A) and standard highGM plus IL-4 conditions (B) for 6 days, stimu...

Proliferative capacity of expanded Treg.

Jorieke H. Peters (270898)
Luuk B. Hilbrands (87735)
Hans J. P. M. Koenen (87732)
Irma Joosten (27492)

February 2013

<p>Treg and Tconv were expanded according the schedule in <a href="http://www.plosone.org/article/in...

Measurement of T-lymphocyte responses in whole blood cultures using newly synthesized DNA

P. R. Sottong
J. A. Rosebrock
J. A. Britz
T. R. Kramer

January 2000

The proliferative response is most frequently determined by estimating the amount of [3H]thymidine i...

Allogeneic mixed lymphocyte reactions.

Hui-feng Yang (300559)
Ze-hua Zhang (5651692)
Liang-bi Xiang (300560)
Kang-lai Tang (300561)
Fei Luo (289384)
Chun-yu Liu (1865890)
Jian-bo Zhou (300562)
Jin-qing Li (300563)
Jian-zhong Xu (5651695)

February 2013

<p>The allostimulatory capacities of the −/+25(OH)D<sub>3</sub>, 1,25(OH)2D<sub>3</sub>(10 pM) and 1...

TIM-3 antibodies do not affect human T cell activation.

Judith Leitner (392966)
Armin Rieger (322069)
Winfried F. Pickl (392967)
Gerhard Zlabinger (392968)
Katharina Grabmeier-Pfistershammer (322059)
Peter Steinberger (392969)

March 2013

<p>CD4 T cells were stimulated with plate-bound antibodies to CD3 and CD28 immobilized at the indica...