Formamide denaturation profiles with conventional target and highly abundant non-target.

<p>The example probe, E751–772, is a perfect match to the 16S rRNA gene of <i>E. coli</i> and has one mismatch to <i>R. sphaeroides</i>. Curves are theoretical predictions fitted to the experimental scale. Eco, <i>E. coli</i>; Rsp, <i>R. sphaeroides</i>.