Growth rate dependence on amidase expression in different <i>E. coli</i> strains.

<p><b>A</b>. Sample Western blot of pJK_proB_amiE expressed in <i>E. coli</i> TUNER. T – total cell lysate, I – insoluble fraction of the cell lysate, S – soluble fraction of cell lysate. <b>B</b>. Quantification of insoluble amiE relative to total amiE expressed for plasmid pJK_proB_amiE expressed in TUNER and in MG1655rph⁺. The percentage of insoluble amiE was determined by Western blots, while the absolute amount of soluble amiE was determined by enzyme assay of total cell lysates. <b>C</b>. Relative growth rate plotted as a function of soluble (open symbols) or total (insoluble plus soluble; closed symbols) amiE mass fraction of d.c.w. for promoter variants grown in M9-CA for <i>E. coli</i> TUNER (orange circles) or MG1655rph⁺ (blue diamonds). Error bars represent one standard deviation of at least three independent measurements. The solid lines represent linear best fits through the combined datasets. The dashed olive lines represent the range of values from theoretical predictions.