Quantification of expression of <i>HMA3</i> in various <i>A. thaliana</i> accessions by quantitative real-time RT–PCR.

<p>A. Expression of <i>HMA3</i> in shoot and root of <i>A. thaliana</i> accession Col-0 and CS28181. B. Correlation between root expression of <i>HMA3</i> and leaf Cd accumulation in 14 <i>A. thaliana</i> accessions. For the analysis <i>UBC</i> (<i>AT5G25760</i>) was used as an internal normalization standard across all samples. The expression of <i>HMA3</i> was calculated as 2^−ΔCT relative to <i>UBC</i>. Data represent means ± standard error (n = 4 independent biological replicates per accession and tissue).