Enrichment of miR-210 and miR-147 family members-predicted targets in the different subsets of modulated transcripts following transfection by each miRNA candidate. A

<p>) Graphs adapted from our webtool miRontop <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044919#pone.0044919-LeBrigand1" target="_blank">[33]</a> showing the significance of the enrichment (represented as –log10 (adjPVal) according to the fold enrichment in experiments of overexpression of miR-210, miR-147a and miR-147b. Enrichment was calculated according to TargetScan, microCosm or a 2–8 seed search in 3′UTR. On each panel, miR-210, miR-147a and miR-147b are highlighted as blue, green and red dots, respectively. <b>B</b>) Venn diagram summarizing the predicted common targets of miR-210 and miR-147 family members using the combination of transcriptomic data and different bioinformatics prediction tools: TargetScan and a search of a 6 nt complementary seed 2–7 sequences in 3′UTR. Cut offs used for the selection of down-regulated transcripts: log2Average>8; logFC<-0.5 and Adj.pVal<0.05.