Identification of miR-27a putative target genes.

<p><b>A)</b> miR-27a over-expressing RH36 cells were used to perform gene expression analysis by microarrays. SAM two-paired analysis identified 3,345 differentially expressed genes between miR-27a overexpressing cells with respect to controls. <b>A</b>) When differentially expressed genes with putative miR-27a targets obtained using the two prediction algorithms mirSVR and TargetScan 5.1 were crossed, a total of 507 genes, including 97 transcripts predicted by both algorithms, were found. <b>B</b>) Functional enrichment analysis was performed using the DAVID annotation tool, and the most enriched biological categories (modified Fisher exact <i>p-value</i> (EASE score) < 0.05) are shown. The area of each functional category (circle) is directly proportional to the number of genes. The complete list of 507 differentially expressed genes according to DAVID analysis is provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0125171#pone.0125171.s001" target="_blank">S1 Table</a>.