Structural model for the PilP C-domain bound to the PilQ N0 domain.

<p>A) Peak attenuation mapped on to the PilP C-domain (PDB accession 2IVW) following titration with N0N1PilQ^343–545. Ratios of PilP:PilQ were colored as follows: 1∶0.1, red; 1∶0.5, orange; 1∶0.8, yellow; 1∶1, pale-yellow. Left, ribbon plot with β1 stand marked; right, surface plot. B) Peak attenuation mapped on to N0PilQ^343–442. Ratios of PilQ:PilP were colored as follows: 1∶0.1, dark-blue; 1∶0.2, blue; 1∶0.3, cyan; 1∶0.5, pale-blue. C) Model of the PilP^77–164: N0PilQ^343–442 complex generated from CNS1.2 <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat.1002923-Brunger2" target="_blank">[56]</a>, with PilP^77–164 in gold and N0PilQ^343–442 in blue. Flexible residues at the N- and C-termini have been removed for clarity. D) Surface plot of the N0PilQ^343–442 domain generated using CONSURF <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat.1002923-Ashkenazy1" target="_blank">[35]</a> and CHIMERA <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat.1002923-Pettersen1" target="_blank">[69]</a>, with the same color scheme as used in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat-1002923-g002" target="_blank">Figure 2B</a>. A ribbon plot of the PilP C-domain structure <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat.1002923-Golovanov1" target="_blank">[29]</a> is shown in green. The same sequence set was used for CONSURF as employed in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002923#ppat-1002923-g002" target="_blank">Figure 2 B</a>).