Modelling and structural analysis of HP1214.

<p>(A) Sequence alignment of HP1214 and its structural template PDB_ID: 1WD5_A. (B) Superimposition of HP1214 model (yellow) with its template structure PDB_ID: 1WD5_A (purple). (C) In the model structure HP1214, CastP predicted cofactor binding pocket is shown in light blue surface (D) Superimposition of the predicted ligand binding site of model with the template structure. (E) Structure based sequence alignment of HP1214 with PDB_ID: 3G6W_D, residues involved in ligand binding are shown by black boxes, residues of the loop region involved in the dimer formation are shown in blue boxes while red boxes indicate residues involved in the formation of inserted loop. (F) Association of the PRPP ligand to the predicted binding site (residues in yellow) based on high similarity to a known PRPP binding site of template which is PDB_ID: 3G6W_D (residues in blue). PRPP is represented in ball and stick model, carbon- green, oxygen- red, nitrogen- blue and phosphorus- orange.