Effect of different caspase inhibitors and caspase 2 silencing on apoptotic death in cells exposed to clofibrate.

<p>(A) cells were pretreated with the indicated inhibitors, exposed to clofibrate (CF) and analysed after 45 min. Histograms refer to percentages of cells with hypodiploid DNA content (black bars: untreated; grey bars: CF-treated). (B) sh-RNA effectively abrogates caspase 2 expression in Jurkat cells. Co = control, Scr = scramble shRNA, shCSP2 = caspase 2-specific shRNA; Lane 1: molecular weight marker. Tubulin is used as loading control. (C) flow-cytometric analysis showing the percentages of cells with hypodiploid DNA content of the above mentioned cells exposed to 1 mM clofibrate for 45 min. Data are expressed as mean ± SD (n = 3). ** p<0,01 vs Co; *** p<0,001 vs Co; §§ p<0,01 vs CF; §§§ p<0,001 vs CF.