Characterization of astrocytes-neurons interplay.

<p>Photos show confocal images of immunoreactivity of GFAP (green) and NeuN (red) in CA1 Pyramidal cell layer and CA1 Str. Radiatum of adult (<b>A</b>), aged (<b>B</b> and <b>D, E1–F3</b>) and LPS-treated rats (<b>C</b>). Scale bar: 60 µm (<b>A</b>,<b>B</b>,<b>C</b>). <b>D:</b> 3D stack of confocal scans of GFAP (green), and NeuN (red). Scale bar: 5 µm. <b>E1–E3:</b> each panel is obtained merging 2 consecutive confocal scans (total 0.738 µm). Scale bar: 5 µm. <b>F1–F3∶</b>3D stacks of the neuron shown in D, digitally cut along the white dotted line and rotated by 0, 45 and 90 degrees along the vertical axis. Scale bar: 3 µm.