<i>Gabrg1</i> expression in proprioceptors in rostro-caudal gradient and regulated by NT3.

<p>(a) <i>In situ</i> hybridization experiments demonstrating downregulation of <i>Gabrg1</i> expression in p0 <i>TrkC</i> mutant L5 DRG when compared to wild-type. (b) Affymetrix expression value analysis of <i>Gabrg1</i> in proprioceptors (TrkC) and non-proprioceptors (nonTrkC) of wild-type (left), <i>mlc^NT3</i> (middle) and <i>NT3^−/−Bax^−/−</i> (right) mice (y-scale displays raw expression values; ±SEM). Note selective upregulation of <i>Gabrg1</i> in proprioceptors of <i>mlc^NT3</i> and downregulation in <i>NT3^−/−Bax^−/−</i> mice compared to wild-type values, in absence of gene expression changes in non-proprioceptors. (c–e) <i>In situ</i> hybridization analysis of <i>Gabrg1</i> expression on p0 wild-type DRG, displaying representative images from lumbar levels L1, L3, L5 and L6 (c) and quantification of cell numbers at lumbar (d) and cervical (e) rostro-caudal levels (n = 3 animals; ±SEM). (f) Affymetrix expression value analysis of <i>Gabrg1</i>, <i>TrkC</i>, and <i>Pvalb</i> at L1 and L5 (y-scale displays raw expression values; ±SEM). (g, h) Analysis of <i>Gabrg1</i> expression in adult DRG by <i>in situ</i> hybridization (g: L1 and L5; scale bar = 80 µm), Affymetrix gene expression values (h; n = 3; ±SEM), and quantitative PCR (h; n = 3; ±SEM). In comparison, Affymetrix expression values of <i>TrkC</i> are not significantly different between L1 and L5 adult DRG (h; n = 3; ±SEM).